EXAM RECALLS wordsology. Many folks have been asking for exam recalls to be emailed to them. Fortunately, your colleagues have previously posted and continue to post recall questions from recent exams. These are located throughout the discussion areas. I continue to compile them all in this area. If you have additional questions to contribute, please do so and you will be credited. Pay it forward and be a good human citizen Thank you Many thanks to King Bego, SS, Zsa, Yohannes, Jord, Clo. Fro, Adrienne Salazar, Kk, Adrienne, Choneng, Oliver A, Aleesha, Yeng, Christian, Caroline, may, Gab, Ipassed, Rufio. SD6. 19, Passit. Forward, student in USA, LM, Ergo, Michi, JB, Veronica, Chellezy. Why Are Serial Dilutions Used In ElisaSniper, CC, Nancy, ariel acaylar, Ginger, ge. Nika, Danny Lyons, Kobe, Valen, Yoro, Dee, Jean, Ruby, Dee. Cee, FM, John, newmt, CG, Forlornd, Diana, Paralumann, K, Charles, Ryan, Nicole, Sekonie, Kbrown, asdfgaill, Itina, Nicole, Klynn, Liv, TB, Jan, Zinnia, Samsam, Sue. S, Saro, Violeta, Sukhi, Maricel, Jamaica, mllerena, Bernadette, Dora. DExplorer, annu, Shiela, RJ Baclagan, Kuki, Kaneulchan, Mr IT, Maria, Violeta, Anonymous, tao tao, Alexis, Krabbypatty, lead, Jordan, miroslavafh, Roela GV, Tazeen. Rondrae, Sal, Sarah, Sam, Jen, Charita, Daniel, liwa. Here are my recalls Steno maltophilia multi drug resistant and maltose fermenter Wilsons Dse confuse between increase CK and ceruplasmin my answer or increase ALT and ceruplasmin. I chose this because of CK BB and mostly lead affects brain functioning. ALT is mainly for liver dse Suspected bioterrorism agent morphology satellitism with Staph aureus Gram stain GNCB. I answered, rule out Francisella and Bordetella because these ar e characteristics of Haemophilus which is a common pathogen P antigen deteriorate over time Mycoplasma pnuemoniae pt did not respond to atb due to lack of cell wall positive and negative controls of bile esculin, salt tolerance, CAMP and one more test I cannot remember FFP thawed at 1. C. Transfusion is due at 3 pm. Not mentioned if pooled FFP expiry is 4 hrs so I answered keep on the fridge and wait for doctors instructions since normal thawed ffp can last for 2. Difference between Pseudo putida and aeruginosa Plasmodia spp. P. falciparum Picture of stomatocytes liver dse Tabular CBC result of method A and B. In method A, WNBC is increased. In method B, WBC is normal. Beside it is a peripheral smear of target cells and Hb C bar shaped. Error in method A is on the lysing reagent Bilirubin metabolism Baby is O using cord blood sample. Mother is AB neg. I answered repeat blood group from heel stick. Ab ID Ab identified is Lea and Leb, but choices are the description or characteristics of Abs so I chose glycolipids adsorb on plasma. Im thinking it should be adsorb on red cells but since no other answer related to adsorption so I answered this one. Many blood bank questions especially DAT but I can hardly remember. Why Are Serial Dilutions Used In Biological Experiments' title='Why Are Serial Dilutions Used In Biological Experiments' />Clostridium botulinum is an anaerobic, rodshaped sporeforming bacterium that produces a protein with characteristic neurotoxicity. Under certain. Genetics RealTime PCR Homepage. Fairfax Financial Holdings Limited Program. Infinity Cdma Activation more. GLOSSARY OF REALTIME PCR TERMS. M. Tevfik Dorak. Address for bookmark httpwww. Why Are Serial Dilutions Used In Biology' title='Why Are Serial Dilutions Used In Biology' />Water memory is the purported ability of water to retain a memory of substances previously dissolved in it even after an arbitrary number of serial dilutions. Batimastat BB94 is a potent, broad spectrum matrix metalloprotease MMP inhibitor for MMP1, MMP2, MMP9, MMP7 and MMP3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and. Reagent preparation Wash Buffer If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely. How to Do Serial Dilutions. A dilution in chemistry is a process that reduces the concentration of a substance in a solution. A serial dilution is the. Frequently Asked Questions Coming soon BAX System, Lateral Flow System, and RiboPrinter System FAQs Do you have a question that is not answered below Ask yo. Homeopathy is a system of alternative medicine created in 1796 by Samuel Hahnemann, based on his doctrine of like cures like similia similibus curentur, a claim. RESOURCES FOR STUDENTS TEACHERS DEADLY EEI IDEAS Ideas for Year 11 and 12 Chemistry Extended Experimental Investigations. From Dr Richard Walding, PhD, FAIP. Review high yield notes and other questionnaires you have. I used this site for review. Other review materials include Polansky, harr, boc and labce. Try to answer all recall questions especially the latest ones, but dont rely always on the answers. Better check it by yourself. If you are sure of your answer, dont hesitate to follow your instincts. I never flagged any question since 2 hrs and 3. I finish my exam within an hour. Study hard and pray always for guidance. All the best for all takers. Thank you for this amazing website, I took Ascpi yesterday and I passed my 1st attempt. Graduated in 2. 01. Medical Laboratory Science. I passed really not because I graduated as a medical doctor few years back but because of Gods divine and unmerited grace and this amazing website. I was guided thoroughly by those recalls and comments from many people on this site. Over half of the questions on my exam were actually either related to the things already discussed here or directly same questions. To God I give all the praises and to all who contributed here I say thank you and may God bless you, and to Sohail I say may God bless you richly and immensely. RECALLS something seen in primary biliary cirrhosis unconjugated bilirubin seen in 2 year old baby with normocytic and normochromic blood picture picture of burr cell and the cause picture of stomatocytes and the cause picture of acanthocytes and the cause know and if possible memorize all the high yield notes and diagrams microbiology. I got over 1. 5 to 2. AHG, IS, 3. 7 characteristics of each especially kell, duffy, mns lewis, and kiddalso anti i and anti I urine reagent strip principles, causes of false positives and false negatives tumor markers acute pancreatitis, breast cancer, hepatic ca, etc PSA for prostate I was given a scenario where one month after surgery PSA was high so what happened so many blood serology questions leukemia and markers transfusion reactions and causes casts, crystals and where they are found hepatitis markers PT AND PTT studies warfarin and heparin diabetes and how to diagnose it, Conns syndrome, Sushing syndrome and the lab values. I had no textbook so I read polansky flash cards, harrs review book tho i didnt finish it bc I had less than two months to prepare. Anyway Im going to share some points. Try to study Harr questions and also BOC for BB. Some of the questions in the BB actual exam were taken from BOC. Try to focus on A bottomline approach by theriot and also the book of ciulla reasons for falsely decinc PT and PTT. Ca affted by PTH Relevance of sodium and glucose T. T. rubrum Hair shaft overdose of salicylate, what chem test is to be tested olive oil M. Degradation of reagent in PTPTT reason for the qc to fail arrange by protein lipid ratio hdl, vldl, ldl, idl i forgot my answer here patient is A positive but no A positive is available only O negative what will you do Burr cells is an indicative of what urine cast will appear in patients with nephrotic syndrome Rbc cell seen in patient with mycoplasma pneumoniae BB remember the abo discrepancies and also the antibody identification. Memorize by heart the high yield notes of Sohail for Enterobacteriaceae and for gram positive cocci and bacilli it can definitely save your life from micro questions. They asked about Stenotrophomonas maltophilia which are Rapid oxidizers of maltose. The asked about the stain used for Cryptosporidium parvum Modified trichrome stains. Here are some questions I remember 1. Markers absent in Acute promyelocytic leukemia CD1. I picked Dont know if its right. Group A, Le ab person Lea only in saliva Because no Leb no secretor gene no A H antigens in saliva3. Burr uremia, Stomatocytes Liver disease, Acanthocytes inadequate slide drying. Picture shown for these so know what these look like under the microscope4. ANA pattern that looked smooth but had orange fluroscence along with green. Anti mitochondrial Dont know if its right5. PT, PTT, and Pt. samples all run together were abnormally high Choices Ca. Cl. 2 added, thromboplastin added, controls deterioration, incubation temp. I picked this one because the others didnt make sense to me6. PT and PTT were high Heparin contamination. FeFeUIBC X 1. Antigen that deteriorates P group. Procainmide toxicity, levels within range, what to do next Repeat test on same sample, Recollect and repeat, Test NAPA levels What I picked, dont know if its right, Test phenobarbital levels. Pall Forte. Bio References in Literature Inhibition of Pax. Transcription Activation with a Small Molecule that Targets the DNA Binding Domain. Grimley E, et al., ACS Chem Biol, 1. The Pax gene family represents crucial embryonic developmental control genes that encode DNA binding transcription factors. In vertebrates, Pax. Authors hypothesize that Pax. Described herein is the use of a homology model of the Pax. Pax. 2 transcription activation by targeting the Pax. DNA binding domain. The EG1 was identified as a compound that can effectively block Pax. Gta Iv 2013 Edition Repack more. DNA binding. Binding affinity and kinetics of EG1 interacting with the Pax. Bio Layer Interferometry BLI with a Pall Forte. Bio Octet RED system. Biotinylated Pax. Streptavidin Biosensor probes. Subsequently, sensor tips were incubated in various concentrations of EGI. The binding buffer contained PBS with 0. DMSO. kon, koff, and KD values were determined by global fitting of the binding curves. A steady state analysis of the binding curves Req. Concentration provided an affinity constant consistent with that obtained from on and off rates. Overall results of this study suggest that the targeting of tissue specific developmental control genes has a significant potential to minimize off target activity and to be more effective in treatments. Pub. Med Vesicular Stomatitis Virus N Protein specific Single domain Antibody Fragments Inhibit Replication. Hanke L, et al., EMBO Rep, 1. Vesicular stomatitis virus VSV single stranded RNA genome is tightly encapsidated by nucleoprotein N to form a nucleocapsid N RNA. N RNA serves as the template for RNA synthesis. In the absence of nucleoprotein N, full length RNAs will not be produced and therefore nucleoprotein N is considered as a target of intervention of the virus life cycle. Described herein is the use of cytosolically expressed variable region of the heavy chain of camelid heavy chain only antibodies VHHs to target the viral nucleoprotein N. Four of the VHHs identified are specific for VSV. Affinity and kinetic parameters were determined by using Bio Layer Interferometry BLI. A Pall Forte. Bio Octet RED9. Streptavidin Biosensor probes was used to perform all BLI experiments. Biosensor tips were loaded with biotinylated WT and escape mutant versions of N RNA variants. Association and dissociation of VHHs were studied over a series of concentrations. Experimental data obtained were fitted using the 2 1 heterogeneous ligand binding global fit model. KD values were determined. The binding sites on nucleoprotein N for some of the identified VHHs were characterized and the information was used to present a molecular explanation for the inhibitory effects of the N specific VHHs. Overall, these findings reveal new features on the N protein surface that may have the potential to be used as an antiviral intervention options. Pub. Med Tumor secreted Anterior Gradient 2 Binds to VEGF and FGF2 and Enhances Their Activities by Promoting Their Homodimerization. Guo H, et al., Oncogene, doi 1. Human anterior gradient 2 AGR2 is a protein that overexpresses in human adenocarcinomas. The exact function of secreted AGR2, including its mechanism of action is not well understood. Reported herein is the mechanism for the tumor promoting function of extracellular AGR2. Furthermore, authors demonstrate that extracellular AGR2 directly binds to vascular endothelial growth factor VEGF and fibroblast growth factor 2 FGF2 and amplify their activities. Binding affinity of AGR2 to various growth factors were studied by using the Bio Layer Interferometry BLI. A Pall Forte. Bio Octet RED9. Ni NTA biosensors was used to perform all BLI experiments. Biosensor tips were immobilized with His AGR2. Subsequently, the AGR2 loaded biosensor tips were dipped in solutions of VEGF1. VEGF1. 65, VEGF1. FGF2, EGF, IGF 1 and TGF1, respectively. KD values were determined for all the binding interactions. Collectively, findings of this investigation suggest that the secreted AGR2 is a promising antitumor target, which affects the function of extracellular signaling networks. Pub. Med Structural Basis for Dimerization and RNA Binding of Avian Infectious Bronchitis Virus nsp. Hu T, et al., Protein Sci, 2. Coronaviruses Co. Vs are positive strand RNA viruses with an ability to infect animals as well as humans. Co. Vs produce nonstructural proteins nsps that play a key role in assembling the RNA replicase complex, which is essential for viral replication and transcription. Nsp. 9 forms a homodimer, and its dimeric form is critical for RNA binding. An investigation directed towards the exploration of the structure and oligomerization state of a gamma Co. V, reported herein is an avian infectious bronchitis virus IBV nsp. The crystal structure is solved for IBV nsp. According to the crystal structure, a hydrophobic region and two parallel helices are vital for nsp. The dimeric state was further studied using various biochemical techniques. Binding interactions of wild type and mutant nsp. RNA were investigated by using Bio Layer Interferometry BLI. A Pall Forte. Bio Octet RED9. Streptavidin SA Biosensor probes were used to perform BLI assays. Biotinylated 2. 0 mer RNA was loaded onto SA Biosensor tips. Sensor tips were immersed in various concentrations of nsp. KD values were calculated for the binding interactions. Data obtained by BLI was confirmed also by Surface Plasmon Resonance SPR method. Overall results of this study suggest that the dimeric form of nsp. RNA. Furthermore, three conserved residues within the dimeric interface of IBV nsp. RNA interactions. Pub. Med Enhancement of Antibody Functions Through Fc Multiplications. Wang Q, et al., Mabs, 93 3. The crystallizable fragment Fc domain of an Ig. G antibody molecule presents an excellent target for protein engineering to develop antibodies with better therapeutic effects. Described herein is the engineering of tandem Ig. G1 Fc molecules by adding one or two extra copies of Fcs and assessing the effects of Fc multiplication or tandem Fc on antibody functions. Furthermore, authors have investigated the functional activities of these newly developed antibodies both in vitro and in vivo. All expressed Ig. G variants were quantified by using Bio Layer Interferometry BLI. A Pall Forte. Bio Octet QK3. Protein A Pro A Biosensor probes was used to perform BLI experiments. Authors have observed a significant enhancement of protection provided by the antibodies with 3 Fc tandem repeats in a Klebsiella pneumoniae mouse therapeutic model. The observations of this study reveal a new Fc engineering technology with an unique ability to expand the development of improved antibody therapeutics. Pub. Med Immunodominant Protein MIP0. Mycobacterium Indicus Pranii Displays Chaperone Activity. Sharma A, et al., FEBS J, 2. Mycobacterium indicus pranii MIP is a nonpathogenic, saprophytic, cultivable organism and it has attracted attention as a potential intervention against many diseases including tuberculosis. Reported herein is the biophysical and biochemical characterization of an immunodominant MIP protein, MIP0. The interaction of MIP0. CS was studied by using the Bio Layer Interferometry BLI platform. A Pall Forte. Bio BLItz instrument equipped with his tagged MIP0. Ni NTA Biosensor probes was used to perform all BLI experiments. CS native or non native was allowed to interact with MIP0. BLI data revealed that MIP0. Overall results of this investigation confirms MIP0.